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  • 我校在水稻籼粳杂种不育分子机理
    11月3日,我校生命科学学院、亚热带农业生物资源保护与利用国家重点实验室在国际著名学术期刊Nature Communications(IF2017=13.0)在线发表了题为“Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice”(基因组结构变异介导的基因等位抑制导致水稻籼粳杂种不育的分子机理)的论文(在线网址:https://www.nature.com/articles/s41467-017-01400-y)。论文第一作者是我校博士后沈荣鑫博士,通讯作者是刘耀光研究员。该论文是我校在阐明控制水稻杂种不育Sa和S1座位分子机理后的又一重大研究成果。 杂种不育是物种分 ...more>>

  • Targeted Genome Editing in Gen
    近日,Molecular Plant杂志在线发表了华南农业大学生命科学学院刘耀光研究组的题为“Targeted Genome Editing in Genes andCis-Regulatory Regions Improves Qualitative and Quantitative Traits in Crops”的论文,系统的论述了利用CRISPR/Cas9(Cpf1)对基因的顺式调控元件进行编辑方式改良作物数量和质量性状的方法和前景。Targeted Genome Editing in Genes andCis-Regulatory Regions Improves Qualitative and Quantitative Traits in CropsXitao Li,Yongyao Xie,Qinlong Zhu,Yao-Guang LiuFig. Strategies for removing and modifying gene function by the genome editing technology.原文如下:http://172.17.0.111/cache/6/03/www.cell.com/56abeb2bc686cf6c8890c767e0a88338/S1674-2052%2817%2930308-8.pdf ...more>>

  • A robustCRISPR/Cas9 system for
    CRISPR/Cas9 targeting systems have been applied to a variety of species. However, most of current CRISPR/Cas9 systems for plants can only modify one or a few target sites.Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon-optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants. We designed PCR-based procedures to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR/Cas9 vectors in one roun ...more>>

  • Rapid Decoding of Sequence-Spe
    The widely applied programmable sequence-specific nucleases, such as TALENs and CRISPR/Cas9, usually induce bi-allelic, heterozygous, and homozygous mutations in plants. Direct sequencing of PCR products containing such heterozygous and bi-allelic mutations results in unreadable superimposed sequencing chromatograms, starting from the mutant site. Here, we present the simple yet highly reliabledegenerate sequence decoding (DSD)method for rapid decoding of suchsuperimposed sequencingchromatograms. DSD will greatly ...more>>

  • The Rice Restorer Rf4 for Wild
    The Wild-Abortive cytoplasmic male sterility (CMS-WA)system is widely used for hybrid rice breeding. Recently, we reported that anovel, chimeric mitochondrial gene, WA352,causes CMS-WA by inducing premature tapetalprogrammed cell death. Two majorrestorer genes, Rf3 and Rf4, control fertility restoration ofCMS-WA. Here we report the cloning and functional examination of Rf4. We mapped Rf4 to a chromosomal region within a cluster of genes encodingpentatricopeptide repeat (PPR) proteins. We then used functionalcomple ...more>>

  • A detrimental mitochondrial-nu
    Plant cytoplasmic male sterility (CMS) resultsfrom incompatibilities between the organellar and nuclear genomes and preventsself pollination, enabling hybrid crop breeding to increase yields. The WildAbortive CMS (CMS-WA) has been exploited in the majority of 'three-line' hybridrice production since the 1970s, but the molecular basis of this trait remainsunknown. Here we report that a new mitochondrial gene, WA352, which originatedrecently in wild rice, confers CMS-WA because the protein it encodes interactswith t ...more>>

版权所有:[华南农业大学][生命科学学院] - 刘耀光实验室 技术支持:广东省东莞市力拓科技有限公司
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