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刘耀光实验室简介

长江学者杰青获得者刘耀光课题组长期从事水稻育性发育分子机理以及水稻基因工程研究,致力于建立功能基因组研究技术平台,分离水稻重要基因特别是育性相关的基因、解析其分子机理和功能,取得多项具有国际领先水平的科研成果。课题组有专职教师5人(研究员1人,副研究员2人,助理研究员1人,实验师1人),博士后、博士研究生、硕士研究30多人,是一支结构合理、学术氛围浓厚、团结和谐、积极进取、特色鲜明的优秀研究队伍。 

主要研究内容

1、水稻细胞质雄性不育及恢复的分子机理

2、水稻核不育的分子机理

3、水稻开花的分子机理

4、植物基因工程载体的开发与应用

科研平台

本科研团队隶属的亚热带农业生物资源保护与利用国家重点实验室和广东省高等学校植物分子生物学与生物技术重点实验室,具备了大型仪器设备如透射电子显微镜、扫描电子显微镜、激光共聚焦显微镜、大型高速冷冻离心机、高效液相色谱仪、超薄切片机、多功能扫描成像系统、基因枪、蛋白质分离系统等。本研究团队具备有常规实验条件,拥有各类离心机、电击转化仪、PCR仪、荧光倒置显微镜、普通显微镜、体视显微镜、摇床、人工气候箱、各类培养箱、植物组织培养室、温室、网室等仪器和设施。

承担教学 

1、参与的本科生课程有

遗传学A、生物化学、分子生物学、植物细胞工程

2、参与的研究生课程有

高级分子生物学、植物细胞工程、基因工程原理与方法、作物遗传育种研究进展、生命科学进展

科研成果

1、科技奖励

(1)杂交稻育性遗传控制的分子机理研究,广东省科学技术奖一等奖(2011年)

(刘耀光,王中华,龙云铭,邹艳姣, 张群宇,赵利锋,李晓瑜,郭晶心,陈远玲,陈乐天, 杨存义,刘振兰, 庄楚雄张桂权,梅曼彤)

2、研究生奖励

(1)全国百篇优秀博士学位论文(2007年 王中华)

3、发明专利

(1)日本发明专利:PCR扩增方法 (TAIL-PCR) (刘耀光)日本专利厅专利号:特开平6-253843

(2)中国发明专利:多基因载体的构建方法与应用 (刘耀光),专利号: ZL02134869.3

(3)中国发明专利:植物细胞质雄性不育恢复基因及其应用 (刘耀光、王中华、吴豪、张群宇、苏弟华、陈乐天),专利号: ZL02152010.0

(4)中国发明专利:一种水稻杂种育性基因及其应用 (刘耀光、杨绍华、陈竹锋、庄楚雄、梅曼彤),专利号: ZL200610011976.4

(5)中国发明专利:水稻杂种花粉育性基因及其应用 (刘耀光、龙云铭、赵利锋、牛百晓、庄楚雄),专利号: ZL200810132341.9

(6)中国发明专利:一种水稻细胞质雄性不育基因及其应用(刘耀光、刘振兰、徐虹、吴豪、郭晶心、张群宇、叶珊、陈远玲),专利号:ZL 200810167024.0

4、代表性论文

1. Zhu Q*, Yang Z, Zhang Q, Chen L, Liu Y* (2014) Robust multi-type plasmid modification based on isothermal in vitro recombination.Gene. 548(1):39-42.

2. Tang H, Luo D, Zhou D, Zhang Q, Tian D, Zheng X, Chen L*, Liu YG* (2014) The Rice Restorer Rf4 for Wild-Abortive Cytoplasmic Male Sterility Encodes a Mitochondrial-Localized PPR Protein that Functions in Reduction of WA352 Transcripts. Molecular Plant. 1497-500.

3. Wang L,  Shen R, Chen L* and Liu YG* (2014) Characterization of a novel DUF1618 gene family in rice. Journal of Integrative Plant Biology. 56 (2):151-158.

4. Chen L and Liu YG. (2014) Male Sterility and Fertility Restoration in Crops. Annu. Rev.Plant Biol. 65: 579-606.

5. Zhang Q,Xue D,Li X,Long Y,Zeng X,Liu Y.2014. Characterization and molecular mapping of a new virescent mutant in rice. J Genet Genomics. ;41(6):353-6.

6. Luo D, Xu H, Liu Z, Guo J, Li H, Chen L, Fang C, Zhang Q, Bai M, Yao N, Wu H, Wu H, Ji C, Zheng H, Chen Y, Ye S, Li X, Zhao X, Li R, Liu YG.A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice. Nat Genet. 2013 45(5):573-7.

7. Yanxiang Jiang, Min Xie, Qinlong Zhu, Xinliang Ma, Xiaoyu Li, Yaoguang Liu, Qunyu Zhang. One-step cloning of intron-containing hairpin RNA constructs for RNA interference via isothermal in vitro recombination system.Planta.2013.(238)325-330. 

8. Wu HB, Wang B, Chen Y, Liu YG*, Chen L*. (2013) Characterization and fine-mapping of the rice premature senescence mutant ospse1. Theoretical and Applied Genetics. 126:1897-1907.  

9. Niu B, He F, He M, Ren D, Chen L*, Liu YG*. (2013) The ATP-Binding Cassette Transporter OsABCG15 Is Required for Anther Development and Pollen Fertility in Rice. Journal of Integrative Plant Biology. 55(8):710-720.

10. Ji C, Li H, Chen L, Xie M, Wang F, Chen Y, Liu YG*. 2013. A novel rice bHLH transcription factor, DTD, acts coordinately with TDR in controlling tapetum function and pollen development. Mol Plant . (2013 );6(5):1715-8.

11. Luo D, Xu H, Liu Z, Guo J, Li H, Chen L, Fang C, Zhang Q, Bai M, Yao N, Wu H, Wu H, Ji C, Zheng H, Chen Y, Ye S, Li X, Zhao X, Li R & Liu YG *. (2013) A Detrimental Mitochondrial-Nuclear Interaction Causes Cytoplasmic Male Sterility in Rice. Nature Genetics, 45:573-577.

12. Chen L*, Wang F, Wang X, Liu YG. (2013) Robust one-tube Ω-PCR strategy accelerates precise sequence modification of plasmids for functional genomics. Plant Cell Physiology, 54:634-642. 

13.  Li R, Xia J, Xu Y, Zhao X, Liu YG*, Chen Y*. Characterization and genetic mapping of a photoperiod-sensitive dwarf 1 locus in rice (Oryza sativa L.). (2013).Theor Appl Genet.127(1):241-50.

14. Chen Y, Liang H, Ma X, Lou S, Xie Y, Liu Z, Chen L, Liu YG *. 2012. An Efficient Rice Mutagenesis System Based on Suspension-Cultured Cells. Journal of Integrative Plant Biology.55:122-130. 

15. Zhou H, Liu Q, Li J, Jiang D, Zhou L, Wu P, Lu S, Li F, Zhu L, Liu Z, Chen L, Liu Y *, Zhuang C *. (2012) Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel non-coding RNA that produces a small RNA. Cell Res. 22:649-660.

16.Ouyang Y, Liu YG and Zhang QF. 2010. Hybrid sterility in plant: stories from rice (应邀综述,封面文章). Current Opinion in Plant Biology, 13(2):186-192.

17. Guo J and Liu YG*. 2009. The genetic and molecular basis of cytoplasmic male sterility and fertility restoration in rice. Chinese Sci Bull, 54(14):2404-2409.

18. Long Y, Zhao L, Niu B, Su J, Wu H, Chen Y, Zhang Q, Guo J, Zhuang C, Mei M, Xia J, Wang L, Wu H and Liu Y-G* . 2008. Hybrid male sterility in rice controlled by interaction between divergent alleles of two adjacent genes. Proc. Natl. Acad. Sci. USA. 105(48):18871-18876.

19. Liu Y-G* and Chen Y (2007) High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. BioTechniques .43, 649-656. Nov;43(5):649-50, 652, 654 passim

20. Liu Z, Xu H, Guo JX, and Liu Y-G* (2007)Structural and expressional variations of the mitochondrial genome conferring the WA type of CMS in rice. J Integr Plant Biol 49 (6), 908-914.

21. Zhang Q, and Liu Y-G* (2006) Rice mitochondrial genes are transcribed by multiple promoters that are highly diverged. J Integr Plant Biol 48 (12), 1473-1477.

22. Chen Y, Zhang Q, Jian Y, Yang Y, Liu K, Liu Y-G* (2006) A rice panicle mutant created by transformation with an antisense cDNA library. J Integr Plant Biol 48 (11), 1300-1305.

23. Wang Z, Zou Y, Zhang Q, Li X, Chen L, Wu H, Su D, Guo J,Chen Y, Luo D, Long Y,Zhong Y, and Liu Y-G* (2006)Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a toxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. The Plant Cell 18: 676 - 687. PMID:16489123

24. Lin Li, Liu Y-G*, Xu X, and Li B. (2003)Efficient linking and transfer of multiple genes by a multigene assembly and transformation vector system. Proc. Natl. Acad. Sci. USA 100: 5962-5967.


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