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刘耀光

点击:次 更新日期:2014年12月15日

个人简介

博士,研究员,博士生导师

中国科学院院士

教育部长江学者特聘教授,“南粤百杰”,

广东省教育厅植物基因组学与生物技术重点实验室主任

(省部共建)教育部水稻育性发育与抗逆重点实验室主任

亚热带农业生物资源保护与利用国家重点实验室副主任

工作经历

1982.3-1984.8 湖南省长沙市农业专科学校,助教

1991.4-1993.3 日本三井植物生物技术研究所,博士后

1993.4-1996.6 日本三井植物生物技术研究所,研究员

1996.7- 现在 华南农业大学生命科学学院遗传工程研究室,研究员

学习经历

学位

学科门类

专业

毕业学校

类型

入学时间

毕业时间

博士

农学

植物遗传学

日本京都大学

全日制

1988-04

1991-03

硕士

农学

作物遗传育种

日本香川大学

全日制

1985-10

1988-03

学士

农学

作物遗传育种

华南农业大学

全日制

1978-03

1982-02

主要研究领域

1、水稻细胞质雄性不育及恢复的分子机理

2、水稻核不育的分子机理

3、水稻开花的分子机理

4、植物基因工程载体的开发与应用 

学术兼职

1、《中国科学.生命科学》,《J Integr Plant Biol》,《J Genetics & Genomics》,《Frontier of Genetics and Gnomics》编委

2、国家自然科学基金评审委员

3、全国遗传学会植物遗传和基因组学专业委员会理事

4、广东省遗传学会理事

5、国家重点实验室(4个)学术委员

荣誉和奖励

1997年度获国家杰出青年基金资助

1999年入选广东省“千百十人才工程”培养对象

2001年入选国家级“百千万人才工程”培养对象

2002年受聘教育部长江学者计划特聘教授

2003年获全国优秀留学回国人员成就奖

2004年获全国五一劳动奖章

2006年获全国师德标兵称号

2011年获广东省科学技术奖一等奖(杂交稻育性控制的分子遗传机理)刘耀光,王中华,龙云铭,邹艳姣,张群宇,赵利锋,李晓瑜,郭晶心,陈远玲,陈乐天,杨存义,刘振兰,庄楚雄,张桂权,梅曼彤

发明专利

1、一种PCR扩增方法 (TAIL-PCR) (刘耀光)日本专利厅专利号:特开平6-253843

2、多基因载体的构建方法与应用 (刘耀光),专利号: ZL02134869.3

3、植物细胞质雄性不育恢复基因及其应用 (刘耀光,王中华,吴豪,张群宇),专利号: ZL02152010.0

4、一种水稻杂种育性基因及其应用 (刘耀光,杨绍华,陈竹锋,庄楚雄,梅曼彤),专利号: ZL200610011976.4

5、水稻杂种花粉育性基因及其应用 (刘耀光,龙云铭,赵利锋,牛白晓,庄楚雄),专利号: ZL200810132341.9

6、一种水稻细胞质雄性不育基因及其应用(刘耀光,刘振兰,徐虹,吴豪,郭晶心,张群宇,叶珊,陈远玲),专利号:ZL 200810167024.0

发表的主要研究论文

1.Zhu Q*, Yang Z, Zhang Q, Chen L, Liu Y* (2014) Robust multi-type plasmid modification based on isothermal in vitro recombination.Gene. 548(1):39-42.doi:10.1016/j.jene.2014..07.004.

2.Tang H, Luo D, Zhou D, Zhang Q, Tian D, Zheng X, Chen L*, Liu YG* (2014) The Rice Restorer Rf4 for Wild-Abortive Cytoplasmic Male Sterility Encodes a Mitochondrial-Localized PPR Protein that Functions in Reduction of WA352 Transcripts. Molecular Plant. 1497-500. doi: 10.1093/mp/ssu047.

3.Wang L,  Shen R, Chen L* and Liu YG* (2014) Characterization of a novel DUF1618 gene family in rice. Journal of Integrative Plant Biology. 56 (2):151-158.doi: 10.1111/jipb.

4.Chen L and Liu YG. (2014) Male Sterility and Fertility Restoration in Crops. Annu. Rev.Plant Biol. 65: 579-606.doi: 10.1146/annurev-arplant-050213-040119.

5.Zhang Q, Xue D, Li X, Long Y, Zeng X, Liu Y.2014. Characterization and molecular mapping of a new virescent mutant in rice. J Genet Genomics. ;41(6):353-6. doi: 10.1016/j.jgg.

6.Niu B, He F, He M, Ren D, Chen L, Liu YG*. 2013. The The ATP-binding cassette transporter OsABCG15 is required for anther development and pollen fertility in rice. Journal of Integrative Plant Biollgy. 2013 Aug;55(8):710-20. 

7. Wu H , Wang B , Chen Y , Liu YG*, Chen L*. 2013. Characterization and fine mapping of the rice premature senescence mutant ospse1.Theor Appl Genet.2013 Jul;126(7):1897-907.

8.Ji C , Li H , Chen L , Xie M , Wang F , Chen Y,  Liu YG*. 2013. A novel rice bHLH transcription factor, DTD, acts coordinately with TDR in controlling tapetum function and pollen development. Mol Plant .2013 Sep;6(5):1715-8.

9.Luo D , Xu H , Liu Z , Guo J , Li H , Chen L , Fang C , Zhang Q , Bai M , Yao N , Wu H , Wu H , Ji C , Zheng H , Chen Y , Ye S , Li X , Zhao X , Li R , Liu YG*. A detrimental mitochondrial-nuclear interaction causes cytoplasmic male sterility in rice.Nat Genet. 2013 May;45(5):573-577.


10.Chen Y, Liang H, Ma X, Lou S, Xie Y, Liu Z, Chen L, Liu YG*. An Efficient Rice Mutagenesis System Based on Suspension-Cultured Cells. Journal of Integrative Plant Biollgy. 2013:55(2):122-130.

11.Li R, Xia J, Xu Y, Zhao X, Liu YG*, Chen Y*. Characterization and genetic mapping of a photoperiod-sensitive dwarf 1 locus in rice (Oryza sativa L.). Theor Appl Genet.2013.

12.Zhou H, Liu Q, Li J, Jiang D, Zhou L, Wu P, Lu S, Li F, Zhu L, Liu Z, Chen L, Liu Y-G*, Zhuang C*. 2012. Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA. Cell Research, (2012):1-12.

13.Ouyang Y, Liu YG and Zhang QF. 2010. Hybrid sterility in plant: stories from rice (应邀综述,封面文章). Current Opinion in Plant Biology, 13(2):186-192.

14.Guo J and Liu YG*. 2009. The genetic and molecular basis of cytoplasmic male sterility and fertility restoration in rice. Chinese Sci Bull, 54(14):2404-2409.

15.Long Y, Zhao L, Niu B, Su J, Wu H, Chen Y, Zhang Q, Guo J, Zhuang C, Mei M, Xia J, Wang L, Wu H and Liu Y-G* . 2008. Hybrid male sterility in rice controlled by interaction between divergent alleles of two adjacent genes. Proc. Natl. Acad. Sci. USA. 105(48):18871-18876.

16.Liu Y-G* and Chen Y (2007) High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. BioTechniques 43, 649-656.

17.Liu Z, Xu H, Guo JX, and Liu Y-G* (2007)Structural and expressional variations of the mitochondrial genome conferring the WA type of CMS in rice. J Integr Plant Biol 49 (6), 908-914.

18.Zhang Q, and Liu Y-G* (2006) Rice mitochondrial genes are transcribed by multiple promoters that are highly diverged. J Integr Plant Biol 48 (12), 1473-1477.

19.Chen Y, Zhang Q, Jian Y, Yang Y, Liu K, Liu Y-G* (2006) A rice panicle mutant created by transformation with an antisense cDNA library. J Integr Plant Biol 48 (11), 1300-1305.

20.Wang Z, Zou Y, Zhang Q, Li X, Chen L, Wu H, Su D, Guo J,Chen Y, Luo D, Long Y,Zhong Y, and Liu Y-G* (2006)Cytoplasmic male sterility of rice with Boro II cytoplasm is caused by a toxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. The Plant Cell 18: 676 - 687.

21.Yang C, Chen Z, Zhuang C, Mei M, Liu Y-G (2004) Genetic and physical fine-mapping of the Sc locus conferring indica-japonica hybrid sterility in rice (Oryza sativa L.). Chinese Science Bulletin, 49 (16) 1718-1721.

22.Liu, Y-G*, ChenY, and Zhang Q. (2004) Amplification of genomic sequences flanking T-DNA insertions by thermal asymetric inlerlaced polymerase chain reaction. In Methods In (Leandro Edited) Molecular Biology Vol. 286. Transgenic Plants Methods and Protocols. pp.341-348.

23.Lin Li, Liu Y-G*, Xu X, and Li B. (2003)Efficient linking and transfer of multiple genes by a multigene assembly and transformation vector system. Proc. Natl. Acad. Sci. USA 100: 5962-5967.

24.Liu Y-G*, Liu H, Chen L, Qiu W, Zhang Q, Wu H, Yang C, Su J. Wang Z, Tian D. and Mei M. (2002) Development of new transformation-competent artificial chromosome vectors and rice genomic libraries for efficient gene cloning. Gene 282:247-255.

25.Shibata D and Liu Y-G, (2000) Agrobacterium-mediated plant transformation with large DNA fragments. Trends in Plant Science 5:354-357.

26.Liu Y-G, Nagaki K, Fujita M, Kawaura K, Uozumi M and Ogihara Y. (2000) Development of an efficient maintenance and screening system for large-insert genomic DNA libraries of hexaploid wheat in a transformation-competent artificial chromosome (TAC) vector. Plant J 23: 687-695.

27.Liu Y-G, Shiraro Y, Fukaki H, Yanai Y, Takasa M, Tabata S and Shibata D. 1999. Complementation of plant mutants with large DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning. Proc. Natl. Acad. Sci. USA 96: 6535-6540.

28. Liu Y-G and Huang N. 1998. Efficienct amplification of insert end sequences from bacterial artificial chromosome clones by thermal asymmetric interlaced PCR. Plant Mol. Biol. Rept. 16: 175-181.

29.Liu Y-G, Mitsukawa N, Liuster C, Dean C and Whittier RF. 1996. Isolation and mapping of a new set of 129 RFLP markers in Arabidopsis thaliana using recombinant inbred lines. Plant J 10:733-736.

30.Liu Y-G, and Whittier, RF. 1995 Thermal asymmetric interlaced PCR: Automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25:674-681.

31.Liu Y-G, Mitsukawa N, Vazquez-Tello A, Whittier RF. 1995. Generation of a high-quality P1 library of Arabidopsis suitable for chromosome walking. Plant J. 7: 351-358.

32.Liu Y-G, Mitsukawa N, Oosumi T and Whittier RF.1995. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8:457-463.

33.Liu Y-G, Whittier RF 1994 Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads. Nucleic Acids Res. 22:2168-2169.

34.Liu Y-G, Mitsukawa N, Whittier RF (1993) Rapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabled sequencing primers. Nucleic Acids Research. 21:3333-3334.

35.Liu Y-G, Ikeda TM, Tsunewaki K(1992) Moderately repeated, dispersed, and highly variable (MRDHV) genomic sequences of common wheat usable for cultivar identification. Theor. Appl. Genet. 84:535-543.

36.Liu Y-G, Tsunewaki K (1991) Restriction fragment length polymorphism (RFLP) analysis in wheat. II. Linkage maps of the RFLP sites in common wheat. Jpn. J. Genet. 66:617-633.

37.Liu Y-G, Mori N, Tsunewaki K (1990) Restriction fragment length polymorphism (RFLP) analysis in wheat. I. Genomic library construction and RFLP analysis in common wheat. Jpn. J Genet. 65:367-380.

38.     吴豪, 刘振兰, 徐虹, 刘耀光* (2007) 植物细胞质雄性不育及其育性恢复的分子基础. 植物学通报24(03):399-413。

39.     胡旭霞,刘耀光*. 植物RNA干涉载体的构建及其在水稻基因表达沉默中的应用 (2006). 分子植物育种 4(5): 621-626.

40.     郭晶心,陈忠正,刘耀光* (2004)水稻抽穗期数量性状基因的定位及遗传效应分析。植物分子育种2(6): 788-794.

41.     杨存义,刘耀光* (2004) cDNA捕捉法获得水稻杂种不育基因座位Sc区域的编码序列。 西北植物学报 24(11):1985-1989。

42.     郭晶心,刘耀光* (2003)水稻籼粳稻杂交F2群体中分子标记得异常分离及染色体定位。分子植物育种 1(3),331-336。

43.     杨存义,陈乐天,陈芳远,刘耀光* (2002) 水稻细胞质雄性不育恢复基系ZSP-1恢复基因的初步定位。 华南农业大学学报 23(4)30-33。

44.     张群宇, 刘耀光*,张桂权,梅曼彤 (2002) 野败型水稻细胞质雄性不育恢复基因Rf4的分子标记定位。 遗传学报 29 (11) 1001-1004。


 



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